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Needle choice generally follows area: finer needles, around 30G to 32G, for facial work such as the chin and jowls, and slightly larger 25G to 27G needles for body areas where more volume needs to be delivered per point

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Post-treatment, the expression of the serotonin reuptake transporter (SERT) significantly increased, while tryptophan hydroxylase-1 (TPH-1) expression significantly decreased in colonic tissue, suggesting exendin-4 mitigates visceral hypersensitivity by upregulating SERT and downregulating TPH-1